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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Topic 1: Blood Banking / Immunohematology- Blood group systems and antibody identification
  • 1. Crossmatching procedures
    • 2. ABO and Rh systems
      Topic 2: Urinalysis and Body Fluids- Fluid analysis and interpretation
      • 1. CSF and other body fluids
        • 2. Urine chemical and microscopic examination
          Topic 3: Hematology- Blood cell analysis and disorders
          • 1. Anemias and leukemias overview
            • 2. CBC and differential interpretation
              Topic 4: Clinical Chemistry- Metabolic and analytical testing
              • 1. Endocrine and metabolic panels
                • 2. Enzymes and electrolytes
                  Topic 5: Microbiology- Clinical microbiological techniques
                  • 1. Culture and sensitivity testing
                    • 2. Bacteriology and organism identification
                      Topic 6: Laboratory Operations- Quality and safety in the clinical lab
                      • 1. Quality control and assurance
                        • 2. Laboratory safety and regulations

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          Question 1

                          n reporting out Gram stains, one should not go beyond what objective observation will allow. In this case, bacterial cells are observed, which are spherical and gram-positive. Their arrangement in tetrads and clusters might be helpful to the physician, suggesting staphylococci. However, one should stop short of naming staphylococci in an official report, as the large gram-positive cocci in tetrads is also suggestive of Micrococcus species, for which human infections have not been reported.
                          A Gram stain of the serous exudate is shown in the image. The appropriate report would read:

                          A. Gram-positive cocci
                          B. Smear positive for bacteria
                          C. Gram-positive cocci in tetrads and clusters
                          D. Gram-positive cocci suggestive of Staphylococcus species


                          Question 2

                          Troponin is a very specific biomarker that is released during cardiac injury or stress. CK is found not only in cardiac tissue, but also in muscle and brain tissue. LD levels can be elevated in cardiac events, tissue breakdown, and hemolysis. Myoglobin is elevated when muscle tissue is damaged and is not specific for the heart muscle.
                          Chemistry
                          Which one of the following is the MOST specific biochemical marker of myocardial infarction?

                          A. Troponin
                          B. CK
                          C. myoglobin
                          D. LD


                          Question 3

                          The most likely causes of delayed hemolytic reactions are Kidd system antibodies. Both jka and jkb are often responsible for delayed hemolytic transfusion reactions.
                          Blood Bank
                          Delayed hemolytic transfusion reactions are usually caused by antibodies directed against what blood group system?

                          A. Kidd.
                          B. Rh.
                          C. ABO
                          D. Kell.
                          E. MNS


                          Question 4

                          A combination of (nonselective) 5% sheep blood and (selective) MacConkey agars is sufficient for the recovery of the pathogenic microorganisms that are most commonly encountered in urinary tract infections (UTIs). MacConkey is the selective culture medium that is most commonly used to inhibit growth of gram-positive organisms (most UTIs are caused by gram-negative organisms).
                          Eosin methylene blue (EMB) is a selective agar that also inhibits the growth of gram-positive organisms.
                          Therefore, using only a combination of MacConkey and EMB would prevent the detection of a gram-positive organism, if this were the cause of the infection.
                          Chocolate agar or other enriched media may be needed in addition to blood and MacConkey if a more fastidious organism is suspected.
                          Thayer-Martin would be used specifically for recovery of Neisseria gonorrhoeae. Thayer-Martin (or Modified Thayer-Martin) inhibits other microorganisms and allows the selective recovery of both N. gonorrhoeae and
                          N. meningitidis.
                          Microbiology
                          Which culture agar combinations below will usually be sufficient for MOST routine urine culture investigations?

                          A. 5% sheep blood and MacConkey
                          B. Thayer-Martin and Chocolate
                          C. 5% sheep blood and Thayer-Martin
                          D. MacConkey and Eosin Methylene Blue
                          E. 5% sheep blood and Chocolate


                          Question 5

                          The creatinine clearance for this patient is 100 mL/min.
                          Creatinine clearance values are calculated using the following equation:
                          Creatinine Clearance (mL / min) = (Urine Creatinine / Serum Creatinine) x Urine Volume (mL) / [ time (hr) x
                          60 ]
                          For this patient Creatinine clearance (mL/min)= (120/1.5) x (1800 / [24 x 60])
                          80 x 1800/1440, which is 80 x 1.25, or 100 mL/min
                          A 45-year-old male of average height and weight had a serum creatinine of 1.5 mg/dL and urine creatinine was
                          120 mg/dL; the total volume of urine collected over a 24-hour period was 1,800 mL. Calculate the creatinine clearance for this patient in mL/min.

                          A. 225
                          B. 177
                          C. 56
                          D. 100
                          E. 144


                          Solutions:

                          Question 1
                          Answer: C
                          Question 2
                          Answer: A
                          Question 3
                          Answer: A
                          Question 4
                          Answer: A
                          Question 5
                          Answer: D

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