Prepare with ASCP ASCP-MLT exam practice material, pass for sure
Updated: Jul 18, 2026
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ASCP ASCP-MLT Exam Overview:
| Certification Vendor: | American Society for Clinical Pathology (ASCP) |
| Exam Name: | MLT(ASCP) Certification Examination |
| Exam Number: | MLT(ASCP) |
| Passing Score: | Scaled score of 400 |
| Exam Duration: | 150 minutes |
| Exam Price: | Approximately USD 200–240 (varies by eligibility route and region) |
| Available Languages: | English |
| Real Exam Qty: | 100 multiple-choice questions |
| Exam Format: | Multiple-choice, Computer-based testing, Single-best answer |
| Certificate Validity Period: | 3 years (ASCP certification maintenance required via continuing education) |
| Related Certifications: | MLS(ASCP) M(ASCP)CM HT(ASCP) PBT(ASCP) |
| Recommended Training: | ASCP BOC Study Materials and Exam Content Guidelines |
| Exam Registration: | ASCP Board of Certification (BOC) application portal |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Computer-based exam administered at Pearson VUE testing centers |
| Pre Condition: | Eligibility requires completion of an accredited MLT program or equivalent education/clinical experience as defined by ASCP BOC routes of qualification. |
| Official Syllabus URL: | https://www.ascp.org/content/board-of-certification/get-credentialed |
ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Objectives |
|---|---|
| Blood Banking / Immunohematology | - Blood group systems and antibody identification
|
| Laboratory Operations | - Quality and safety in the clinical lab
|
| Clinical Chemistry | - Metabolic and analytical testing
|
| Microbiology | - Clinical microbiological techniques
|
| Hematology | - Blood cell analysis and disorders
|
| Urinalysis and Body Fluids | - Fluid analysis and interpretation
|
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Infection early in the pregnancy is the most dangerous time period for the manifestation of anomalies due to rubella. In fact, defects are rare when infection occurs after 20 weeks gestation.
Immunology
The greatest risk for the manifestation of anomalies in maternal rubella infection during gestation is the:
A) second trimester
B) eighth month
C) first trimester
D) delivery
E) third trimester
2. What is the cell that is indicated by the arrow in this field?
A) Red cell fragment
B) Burr cell
C) Acanthocyte
D) Crenated cell
3. The division of Department of Health and Human Services (DHHS) responsible for protecting public health by ensuring the safety and efficacy of foods, drugs, biological products, medical devices, and cosmetics is:
A) the Joint Commission
B) Food and Drug Administration (FDA)
C) Clinical and Laboratory Standards Institute (CLSI)
D) Health Insurance Portability and Accountability Act (HIPPA)
4. Spirochetes such as Borrelia and Treponemes are best visualized using darkfield microscopy. Borrelia, which causes Relapsing fever, can be visualized under darkfield microscopy of wet preps of peripheral blood.
Treponema can be visualized by darkfield microscopy of early primary lesions, and aspirates of affected lymph nodes.
Which of the following organisms is best visualized by use of a darkfield microscope:
A) Entamoeba
B) Streptococcu
C) Campybacter
D) Borrelia
5. Though automated extraction machines have many benefits over manual methods the costs are high and generally require a high throughput of samples in order to justify the costs.
Automated extraction has many benefits over the traditional manual methods. The most important benefit is that the nucleic acid isolated is constantly consistent. There is a reduced amount of manipulation with dramatically decreases the chance of cross contamination. Also, automated extraction machines are considered moderate complexity and can be performed by a wider variety of laboratory professionals.
All of the following are considered benefits of automated isolation and extraction equipment EXCEPT:
A) Automated isolation equipment is cheaper and more practical
B) Automated isolation equipment requires less manipulation
C) Automated isolation equipment is considered moderate complexity
D) Automated isolation equipment provides consistent results
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: A | Question # 3 Answer: B | Question # 4 Answer: D | Question # 5 Answer: A |
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